The action of aminobenzylthiazolium salts on thiamine destruction by the Chastek paralysis enzyme.

Thiamine-modifying properties of fish and meat products.

The presence of antithiamine factors in fish has been known since the early 1940's when it was noted that foxes fed raw carp developed polyneuritic symptoms or Chastek paralysis which was characteristic of thiamine deficiency (1). This condition could be pre vented by heating the fish or including ad ditional thiamine in the diet and was later shown to be caused by the presence of an enzyme "th...

Mechanism-Based Studies of the Active Site-Directed Inhibition and Activation of Enzyme Transketolase

Derivatives of phenyl-keto butenoic acids have been reported to be inhibitors of pyruvate decarboxylase, (PDC). The inhibition of transketolase, a thiamine requiring enzyme such as PDF, by meta nitrophenyl derivative of 2-oxo-3-butenoic acid (MNPB) is reported here. These studies indicate that the inhibitor binds to the enzyme at the active site. A two-step inhibition was observed, first th...

The thermal destruction of vitamin B(1): 4. The influence of the concentration of buffer salts on the rate of destruction of aneurin at 100 degrees.

PHARMACOLOGICAL STUDIES ON NOVEL BASIC AMINOALKYL ARYL ETHERS AS POTENTIAL LOCAL ANAESTHETICS

Following our previous investigations on certain new aminoalkyl aryl ethers, which showed promising and outstanding local anaesthetic properties, the local anaesthetic activity and duration of action of a further selection of four novel derivatives of the above-mentioned series, used as hydrochloride salts, have been determined by the ill vivo rat sciatic nerve test. These are: N,N-dimethyl...

Interactions of Atabrine

The observation that salts of the alkaline earth metals reverse the inhibitory effects of atabrine in the growth of Escherichia coli (1) suggested, as one possibility, that the inhibitor acts by forming complexes with metal-catalyzed enzyme systems. The effect of atabrine on yeast carboxylase was therefore investigated, but the results show no evidence for complex formation involving atabrine a...